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KMID : 0861020080230020051
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Korea Journal of Herbology
2008 Volume.23 No. 2 p.51 ~ p.58
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Effects of Aqueous Extract from Lonicera Japonica Flower on Trimellitic Anhydride-induced Contact Hypersensitivity in BALB/c Mice
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Lee Jung-No
Jeong Seung-Il
Jang Seon-Il
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Abstract
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Objectives: Trimellitic anhydride (TMA), a sensitizer that induces occupational asthma and atopic
dermatitis, is widely used industrially to make epoxy and alkyd resins, plasticizers, high temperature
polymer, and surfactants. The aim of this study was to investigative the effects of aqueous extracts of
Lonicera japonica Flower(LJFAE) on TMA-induced contact hypersensitivity (CHS) in Balb/c mice.
Methods: The dried flowers of L. japonica were extracted with distilled water at 100°C for 7 h. The
extract was freeze-dried following filteration through 0.45 μm filter. Mice were orally administrated
with or without LJFE of a different doses(25-100 mg/kg) for 28 days. In the challenge period, mice
were externally applied at difference doses of LJFAE one time per day 30 min before TMA treatment.
We examined the effects of LJFAE on the the serum levels of IgE and prostagladin E2 (PGE2), the
Th1/Th2 cytokine production of spleen cells, ear swelling responses, and the leukocyte infiltration
induced by TMA.
Results: The orally and externally administration of LJFAE dose-dependently reduced the serum levels
of IgE and PGE2 production as well as ear swelling responses and leukocyte infiltration in
TMA-induced Balb/c mice. Furthermore, the levels of Th1 (TNF-α, IFN-γ, IL-2)/Th2 (IL-4, IL-5,
IL-13) cytokine production from spleen cells stimulated with anti-CD3 and CD28 mAbs was markedly
suppressed by the orally and externally treatment with LJFAE in a concentration dependent manner.
Conclusions: These results suggest that LJFAE suppresses the inflammatory mediators and regulates
the Th1/Th2 cytokines. Therefore, these properties may contribute to the strong anti-CHS response
effect of LJFAE
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KEYWORD
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Lonicera japonica Flower, IgE, Trimellitic anhydride, Th1/Th2 cytokines, Prostaglandin E2, Leukocytes, Contact hypersensitivity, Ear swelling responses
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